Carrier proteins for fusion expression of antimicrobial peptides inEscherichia coli
نویسندگان
چکیده
منابع مشابه
BIOTECHNOLOGICALLY RELEVANT ENZYMES AND PROTEINS Expression of chondroitin-4-O-sulfotransferase inEscherichia coli and Pichia pastoris
Chondroitin sulfates are linear sulfated polysaccharides called glycosaminoglycans. They are important nutraceutical and pharmaceutical products that are biosynthesized through the action of chondroitin sulfotransferases on either an unsulfated chondroitin or a dermatan polysaccharide precursor. While the enzymes involved in the biosynthesis of chondroitin sulfates are well known, the cloning e...
متن کاملHuman Antimicrobial Peptides and Proteins
As the key components of innate immunity, human host defense antimicrobial peptides and proteins (AMPs) play a critical role in warding off invading microbial pathogens. In addition, AMPs can possess other biological functions such as apoptosis, wound healing, and immune modulation. This article provides an overview on the identification, activity, 3D structure, and mechanism of action of human...
متن کاملAcne Inversa: Evaluating Antimicrobial Peptides and Proteins
BACKGROUND Acne inversa is a chronic, suppurative relapsing inflammatory skin disease that primarily affects the axillae, perineum and inframammary regions. Evidence suggests that the innate immune system is involved in the pathogenesis of acne inversa. OBJECTIVE To investigate the role of the innate immune system in acne inversa. METHODS Skin biopsies were obtained from inflammatory skin l...
متن کاملAntimicrobial Peptides Derived from Goat’s Milk Whey Proteins Obtained by Enzymatic Hydrolysis
In this study the bacterial growth inhibitory activity of peptide fragments produced from goat’s milk whey proteins by enzymatic hydrolysis using trypsin, ficin and a combination of both was investigated. Goat’s milk whey proteins were isolated and subjected to enzymatic hydrolysis and peptides were purified by ultrafiltration followed by reverse-phase high-performance liquid chromatography (RP...
متن کاملusing rice flour for purification of maltose binding fusion proteins expressed in escherichia coli
background one disadvantage of expressing heterologous proteins in escherichia coli is that the proteins are frequently expressed as insoluble inclusion bodies. to avoid this problem, heterologous proteins are typically expressed as a fusion protein. maltose binding protein (mbp) is one of the widely used partners for production of recombinant fusion proteins in e. coli. mbp is among the most e...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biotechnology and Applied Biochemistry
سال: 2009
ISSN: 0885-4513,1470-8744
DOI: 10.1042/ba20090087